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upright fluorescence microscope  (Nikon)


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    Structured Review

    Nikon upright fluorescence microscope
    Upright Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c1+microscope/C2%2B/pmc12966746-159-5-8
    Average 99 stars, based on 1 article reviews
    upright fluorescence microscope - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Sevoflurane Postconditioning Ameliorates Neuronal Migration Disorder Through Reelin/Dab1 and Improves Long-term Cognition in Neonatal Rats After Hypoxic-Ischemic Injury.
    Article Snippet: .. Representative images were photographed with a Nikon C1 microscope by a skilled pathologist who was blinded to the experimental conditions and groups. ..

    Article Title: Comparison of bacterial carbon production estimates from dilution and <scp><sup>3</sup>H</scp>‐leucine methods across a strong gradient in ocean productivity
    Article Snippet: Defrosted samples were filtered onto 25-mm polycarbonate filters of 0.22-μm pore size backed by 0.45 Millipore filters. .. The filters were dried at room temperature, and mounted using VECTASHIELD with DAPI (40,6-diamindino2-phenylindole) staining to highlight cell DNA, and imaged at 100X magnification on a Nikon C1 microscope. .. Images were analyzed with Nikon Advanced Research 3.2 software, and either 20 image fields or 200 cells filter 1 were measured for lengths and widths with signal thresholding and length line cutoffs of 0.2–2.0 μm to exclude nonbacteria cells.

    Article Title: A Mitochondrial Kinase Complex Is Essential to Mediate an ERK1/2-Dependent Phosphorylation of a Key Regulatory Protein in Steroid Biosynthesis
    Article Snippet: .. Coverslips were mounted onto the slides using Fluorsave antifade reagent (Calbiochem), followed by confocal analysis using a Nikon C1 microscope (IByME, UBA, Argentina). ..

    Article Title: Multiple sevoflurane exposures during mid‐trimester induce neurotoxicity in the developing brain initiated by 15 LO 2‐ M ediated ferroptosis
    Article Snippet: .. One to two random field of view were obtained on each slide under the 400× field of view of a Nikon C1 microscope. .. IOD values were analyzed by ImageJ.

    Article Title: WDFY3 mutation alters laminar position and morphology of cortical neurons
    Article Snippet: .. To assess genotype distribution and laminar positioning, fluorescent confocal microscopy and image acquisition was performed on an inverted Nikon C1 microscope with associated software, typically at 10 × magnification. .. Images used for cell morphological analysis, including dendritic arbor and spine analysis, were obtained on a Nikon A1 microscope with associated software.

    Article Title: FOXO3 Regulates Sevoflurane-Induced Neural Stem Cell Differentiation in Fetal Rats.
    Article Snippet: With the increase in fetal surgeries, the effect of maternal anesthesia on progeny has attracted much attention.. Our previous studies have demonstrated that 3.5% sevoflurane maternal exposure resulted in over-activated autophagy and cognitive impairment in the offspring.. The autophagy activation resulted in increased apoptosis and decreased proliferation.

    Staining:

    Article Title: Comparison of bacterial carbon production estimates from dilution and <scp><sup>3</sup>H</scp>‐leucine methods across a strong gradient in ocean productivity
    Article Snippet: Defrosted samples were filtered onto 25-mm polycarbonate filters of 0.22-μm pore size backed by 0.45 Millipore filters. .. The filters were dried at room temperature, and mounted using VECTASHIELD with DAPI (40,6-diamindino2-phenylindole) staining to highlight cell DNA, and imaged at 100X magnification on a Nikon C1 microscope. .. Images were analyzed with Nikon Advanced Research 3.2 software, and either 20 image fields or 200 cells filter 1 were measured for lengths and widths with signal thresholding and length line cutoffs of 0.2–2.0 μm to exclude nonbacteria cells.

    Fluorsave:

    Article Title: A Mitochondrial Kinase Complex Is Essential to Mediate an ERK1/2-Dependent Phosphorylation of a Key Regulatory Protein in Steroid Biosynthesis
    Article Snippet: .. Coverslips were mounted onto the slides using Fluorsave antifade reagent (Calbiochem), followed by confocal analysis using a Nikon C1 microscope (IByME, UBA, Argentina). ..

    other:

    Article Title: Definition of mechanisms of viral protein multifunctionality reveals key roles of ‘conformer diversity’ and RNA interaction in forming host interfaces
    Article Snippet: Secondary antibodies (1:1000 dilution) used for 655 IF were goat anti-rabbit Alexa Fluor 568 (Cat #A-11011) and goat anti-mouse Alexa Fluor 568 656 (Cat #A-11004), purchased from Thermo Fisher Scientific.

    Confocal Microscopy:

    Article Title: WDFY3 mutation alters laminar position and morphology of cortical neurons
    Article Snippet: .. To assess genotype distribution and laminar positioning, fluorescent confocal microscopy and image acquisition was performed on an inverted Nikon C1 microscope with associated software, typically at 10 × magnification. .. Images used for cell morphological analysis, including dendritic arbor and spine analysis, were obtained on a Nikon A1 microscope with associated software.

    Software:

    Article Title: WDFY3 mutation alters laminar position and morphology of cortical neurons
    Article Snippet: .. To assess genotype distribution and laminar positioning, fluorescent confocal microscopy and image acquisition was performed on an inverted Nikon C1 microscope with associated software, typically at 10 × magnification. .. Images used for cell morphological analysis, including dendritic arbor and spine analysis, were obtained on a Nikon A1 microscope with associated software.



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    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
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    Image Search Results


    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Journal: Biofilm

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    doi: 10.1016/j.bioflm.2025.100335

    Figure Lengend Snippet: Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Article Snippet: After five days of static incubation at 30 °C, three-dimensional biofilm structures were observed using an Eclipse Ti-E C2+ confocal laser scanning microscope (Nikon) equipped with CFI Plan Apo VC 20x/1.2 and CFI Plan Apo VC 60x/1.2 WI objective lenses.

    Techniques: Staining, Mutagenesis, Fluorescence, Microscopy

    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Journal: Biofilm

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    doi: 10.1016/j.bioflm.2025.100335

    Figure Lengend Snippet: Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Article Snippet: The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope.

    Techniques: Staining, Mutagenesis, Fluorescence, Microscopy